. Bulletin. Agriculture. ACTION ON LEAD ACETATE. 81 deposit fonned was only iil)out one-half the bulk of tlio deposit in (lie pejjtone-niannite tubes. It was of a drab color, eorrespondinj;- v(>ry elosely to Ridgvvay's Ecru Drab, or a little darker than Saccai-do's AvellantHis, No. 7, Talde I, when viewed by reflected li«>ht. The reac- tion of the contents of the tubes was slightly alkaline to litmus at the close of the experiment. The peptone-dextrose tubes began to dear in from ten to twelve weeks after inocula- tion, and m twenty weeks were entirely clear. A large part of the sediment
. Bulletin. Agriculture. ACTION ON LEAD ACETATE. 81 deposit fonned was only iil)out one-half the bulk of tlio deposit in (lie pejjtone-niannite tubes. It was of a drab color, eorrespondinj;- v(>ry elosely to Ridgvvay's Ecru Drab, or a little darker than Saccai-do's AvellantHis, No. 7, Talde I, when viewed by reflected li«>ht. The reac- tion of the contents of the tubes was slightly alkaline to litmus at the close of the experiment. The peptone-dextrose tubes began to dear in from ten to twelve weeks after inocula- tion, and m twenty weeks were entirely clear. A large part of the sediment clung to the back of the upright part of the tube instead of settling completely, as in the other inoculated tubes. The color of the deposit was also drab, corresponding very closely to Kidgway's Ecru Drab, No. 21, Plate III, or a little darker than Saccardo's Avellaneus, No. 7, Table 1, when seen by reflected light. The reaction of the con- tents of the tube at the close of the experi- ment was slightly acid to litnuis. The cane sugar, milk sugar, and glycerin tubes cleared in from one to six weeks. The o-lvcerin tube cleared first, then the milk- sugar tube, and lastly the cane-sugar tu))es. The deposit was heaviest—about -i nnn. deep—in the cane-sugar tubes, about 2 mm. deep in the milk-sugar tubes, and only 1 mm. deep in the glycerin tube. The color of the deposit was the same as in the other cases, viz, Ridgway's Ecru Drab, No. 21, Plate III, or a little darker than Saccardo's Avellaneus, No. 7, Table I. Each inoculated tube gave an acid reaction with litmus at the close of the experiment. No gas formed in any of the tubes. It is therefore apparent that the calla-rot organism is not capable of splitting up mannite, maltose, dextrose, cane sugar, milk sugar, or gh^cerin so that a gas will Fig. 6.—Fermentation tube ten days after inoculating with the callii organism. ACTION ON LEAD ACETATE. Slant tubes of lactose agar, colored with litmus, were inoculated with
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