. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. HYDRAMOEBA AND CHLOROHYDRA 323 to significant differences in hud production during the first two cumulative days of the experiment when averaged over all levels of temperature. The interaction between temperatures and infection, which is not significant, indicates that these two factors are independent; that is, any simple effect of one factor on budding is not dependent upon the level of the other. It should he noted that no host mortality occurred in any treatment combina- tion during the first two clays of this experiment


. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. HYDRAMOEBA AND CHLOROHYDRA 323 to significant differences in hud production during the first two cumulative days of the experiment when averaged over all levels of temperature. The interaction between temperatures and infection, which is not significant, indicates that these two factors are independent; that is, any simple effect of one factor on budding is not dependent upon the level of the other. It should he noted that no host mortality occurred in any treatment combina- tion during the first two clays of this experiment, and that the differences in budding rate, therefore, are due only to the effects of the hydramoebae. To determine how these two factors continue to affect the production of buds during the remaining cumulative time-intervals of the experiment, similar 4OO - 300- o h- o O INFECTION • TEMPERATURE INTERACTION. 200- 1-2 4 1-6 CUMULATIVE DAYS 1-8 1-10 FIGURE 2. Change in the value of F from the analyses of variance of temperature and initial infection effects on bud production. An analysis was performed for each cumulative successive two-day period up to the total of 10 days. The only value of F which is not statistically significant (P-=) is the interaction between temperature and infection during the cumulative first two days of the experiment. analyses of variance were carried out on total bud production by each host for each subsequent cumulative two-day period; that is, from the start of the ex- periment to the end of the fourth day, from the start to the end of the sixth day, and so on, to the end of the tenth day. This procedure involved four more analyses. The changes in the magnitude of the differences in the effects of the twro factors acting separately and in combination are represented by the values of F and are given in Figure 2. Both temperature and infection continued to produce significant effects on the production of buds when examined as single factors. In


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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology