. Bacteria in relation to plant diseases. Bacteriology; Plant diseases. WILT OP CUCURBITS. 287 thinner, according to age, culture-medium, and kind of stain used. Flagella-stains in par- ticular, aflfect an outer part not stained by ordinary methods (figs. 83 and 84). The organism has a distinct capsular portion, the solution of which appears to give rise to the viscidity. This viscidity occurs during active growth, and may continue for some time. When taken directly from the plant (fig. 52) this bacillus is usually .viscid but not always. Often with care the slime may be stretched out to the d
. Bacteria in relation to plant diseases. Bacteriology; Plant diseases. WILT OP CUCURBITS. 287 thinner, according to age, culture-medium, and kind of stain used. Flagella-stains in par- ticular, aflfect an outer part not stained by ordinary methods (figs. 83 and 84). The organism has a distinct capsular portion, the solution of which appears to give rise to the viscidity. This viscidity occurs during active growth, and may continue for some time. When taken directly from the plant (fig. 52) this bacillus is usually .viscid but not always. Often with care the slime may be stretched out to the distance of 20 to 40 cm. (once 76 cm.). The resulting cobwebby threads generally yield pure cultures of this organism and when stained on a slide and examined under a microscope are seen to be made up of bacilli embedded in a tenuous slime which separates them by considerable intervals (Vol. I, figs, 13 and 14). The. Fig. 81.* organism is also sticky, at least in some of the stages of its growth, on agar, gelatin, potato, carrot, sweet potato, coconut-flesh and various other solid culture-media. In one instance the slime from a potato-culture was drawn out 53 cm. before it gave way. On potato, up to the sixth day and sometimes longer, the organism is actively motile, even when examined from very viscid cultures. Potato cultures 10 to 18 days old are usually as sticky as younger ones. This slime does not dissolve readily in water and hence failure may occur in making poured plates. Cultures in potato-broth and in sugared fluids finally become ropy, and then most of the individuals or all of them are dead. *FiG. 81.—Cross-section of one bundle of fig. 80. At bottom and left-hand side are numerous intercellular spaces occupied by bacteria. The bundle has been hollowed into a cavity, and at x, and y parenchyma cells are also occupied by the bacteria. These can be followed through a whole series of sections, but the method of entrance into these cells has not been made out clearly.
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