. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. FIGURE 7. Phase contrast (a, c) and fluorescence (b, d) micrographs of Spisula solidissima meiotic spindles, (a, b) The spindles were incubated at 37°C with DTAF labeled microtubule proteins at mg/ ml. Note the distinct, linear fluorescence, (c, d) 3 X 10~3 M calcium has been added to the fluorescent spindles; note the loss of linear fluorescence (d) and the alteration in spindle morphology in phase contrast (c). Bar = 20 urn. 1. Moreover, the fluorescent proteins retain their native characteristics with respect to sever
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. FIGURE 7. Phase contrast (a, c) and fluorescence (b, d) micrographs of Spisula solidissima meiotic spindles, (a, b) The spindles were incubated at 37°C with DTAF labeled microtubule proteins at mg/ ml. Note the distinct, linear fluorescence, (c, d) 3 X 10~3 M calcium has been added to the fluorescent spindles; note the loss of linear fluorescence (d) and the alteration in spindle morphology in phase contrast (c). Bar = 20 urn. 1. Moreover, the fluorescent proteins retain their native characteristics with respect to several criteria. For example, the modified proteins assemble with kinetics indis- tinguishable from controls and appear morphologically normal when examined in negatively stained preparations (see Figs. 2-4). In addition, the fluorescent microtubule protein is capable of assembly in association with isolated spindles in vitro and can be visualized and photographed using conventional fluorescence microscopy (Fig. 7).. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
