. Fungous diseases of plants : with chapters on physiology, culture methods and technique . Fungi in agriculture. ISOLATION AND PURE-CULTURE METHODS 19 spores, and consequently they may not germinate between the successive sterilizations. On the other hand, if the medium is deep in the vessel, and the exposed surface of the medium' small, much less oxygen gains access, and the spores of anaerobic forms pass more readily into the vegetative condition and are killed by the successive sterilizations. Sterilization under pressure. A great time-saving convenience in sterilization is to be found in
. Fungous diseases of plants : with chapters on physiology, culture methods and technique . Fungi in agriculture. ISOLATION AND PURE-CULTURE METHODS 19 spores, and consequently they may not germinate between the successive sterilizations. On the other hand, if the medium is deep in the vessel, and the exposed surface of the medium' small, much less oxygen gains access, and the spores of anaerobic forms pass more readily into the vegetative condition and are killed by the successive sterilizations. Sterilization under pressure. A great time-saving convenience in sterilization is to be found in the use of the autoclave, or steam. Fig. 5 j5. Autoclave, Horizontal Type, connected with Steam Pipes (All steam apparatus under a hood) pressure sterilizer, two types of which are shown in Fig. 5, a and b. The autoclave is not only more effective than the ordinary steam sterilizer, but by using it the delay of discontinuous sterilization is avoided. In this apparatus the steam is confined, up to any pres- sure desired, instead of being allowed to escape, as in the ordinary steam sterilizer. A good steam pressure gauge on the autoclave is requisite, and a thermometer is not only desirable, but also an additional safeguard. The temperature ordinarily employed is 115,J to 125" C, or about 10 to 20 lbs. pressure. A single incubation. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Duggar, Benjamin M. (Benjamin Minge), 1872-1956. Boston ; New York : Ginn
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