. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 158 MELVIN W. THORNER. the time the fish were taken from the ocean. The eggs were then immediately fertilized by a speim suspension milked from the males. The egg stock thus prepared was kept at room tempera- ture (22-24° C.) in sea water changed daily. At the end of the fourth day after fertilization the heart nor- mally begins to pulsate. All experiments were carried out at room temperature (22-24° C.). The heart was considered as " stopped " when all three chambers had ceased pulsating. EXPERIMENTS. (a) Recovery
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 158 MELVIN W. THORNER. the time the fish were taken from the ocean. The eggs were then immediately fertilized by a speim suspension milked from the males. The egg stock thus prepared was kept at room tempera- ture (22-24° C.) in sea water changed daily. At the end of the fourth day after fertilization the heart nor- mally begins to pulsate. All experiments were carried out at room temperature (22-24° C.). The heart was considered as " stopped " when all three chambers had ceased pulsating. EXPERIMENTS. (a) Recovery in Sea Water. The first set of experiments represents an attempt to determine the relationship between the time of immersion in KC1 and the time of recovery in sea water. Also different concentrations of KC1 were tried to see if the relationship varied for different strengths. For this purpose lots of from 40-75 eggs were chosen for each experiment. The heart of each embryo was observed to be in good physiological condition. Each lot was placed in a 22. 18. lOj 6_ 2.,. RECOVERY INSEAWATER-HRS 10 40 I 50 i 70 20 30 40 50 60 FIG. I. Shows relation between period of recovery in sea water and period of immersion in different strengths of potassium chloride for hearts of Fundulus Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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