. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 394 BARBARA B. NORTH AND GROVER C. STEPHENS i -to 5-10 1 10 5 10' 1 10 1 • 10. No Nit rogen 100 200 TIME (hours) 300 FIGURE 2. Platymonas growth curves. Equivalent weights of nitrogen (30 ) as glycine, K NOS, and (NHi)« HPO4 were supplied. Cell number was determined colorimetrically. centration is presented as Figure 4. This is equivalent to a Lineweaver-Burk plot and permits estimation of maximum velocity from the intercept of the line. The apparent maximum velocity is X 10~10 moles of glycine/minute/5 X 105 cells


. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 394 BARBARA B. NORTH AND GROVER C. STEPHENS i -to 5-10 1 10 5 10' 1 10 1 • 10. No Nit rogen 100 200 TIME (hours) 300 FIGURE 2. Platymonas growth curves. Equivalent weights of nitrogen (30 ) as glycine, K NOS, and (NHi)« HPO4 were supplied. Cell number was determined colorimetrically. centration is presented as Figure 4. This is equivalent to a Lineweaver-Burk plot and permits estimation of maximum velocity from the intercept of the line. The apparent maximum velocity is X 10~10 moles of glycine/minute/5 X 105 cells. The straight line suggests that the rate of uptake is limited by an absorptive step at high concentrations. The process of accumulation of radioactivity in the form TABLE I Uptake of amino acids by suspensions of 2 X 106 cells per nil. Per cent of substrate accumulated after 30 minutes is shown at substrate concentrations ranging from 1 X 10~6 to 2 X 10~7 molar % Accumulation Arginine >95 Glutamic acid 65 Glvcine >95 Phenylalanine 50 Valine 90 Glucose 4. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory


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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology