. Pathogenic micro-organisms, including bacteria and Protozoa; a practical manual for students, physicians and health officers. Contact smear of colony of cholera spirilla Cholera spirilla preparation from gelatin- rom agar. X 700 diameters. (Dunham.) plate culture of cholera. X 800 diameters. the most reliable staining agent with the application of a few minutesheat. It is decolorized by Grams method. The organisms exhibitone long, fine, spiral flagellum attached to one end of the rods, or, ex-ceptionally, to both ends. (Cholera-like spirilla often have 1, 2, or3 end flaffelfa.) In sections t
. Pathogenic micro-organisms, including bacteria and Protozoa; a practical manual for students, physicians and health officers. Contact smear of colony of cholera spirilla Cholera spirilla preparation from gelatin- rom agar. X 700 diameters. (Dunham.) plate culture of cholera. X 800 diameters. the most reliable staining agent with the application of a few minutesheat. It is decolorized by Grams method. The organisms exhibitone long, fine, spiral flagellum attached to one end of the rods, or, ex-ceptionally, to both ends. (Cholera-like spirilla often have 1, 2, or3 end flaffelfa.) In sections thev are stained best bv alkaline methv-lene-blue solution and washed in water slightly acidulated with aceticacid. Biology.—An aerobic (facultative anaerobic), liquefying, verymotile spirillum. Grows readily in the ordinary culture media,best at 37° C, but also at room temperature (22° C.); does not growat a temperature above 42° or below S° C, and does not form spores. In gelatin-plate cultures at 22° C. the colonies are quite character-istic; at the end of twenty-four hours, small, round, yellowish-whiteto yellow
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