. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 21-5K 14-3 K - 6-5K - 3-4K Figure 6. Immunoblot of SDS-PAGE of hemolymph from snails (left lane). The hemolymph samples from a mixed population of snails of known growth rates were subjected to SDS-gel electrophoresis and the fractionated proteins were subsequently transferred to nitrocellulose membrane. The membranes were incubated with the primary antiserum (anti-bovine insulin, 1:1000 dilution) for I h, rinsed in phosphate buffered saline, pH , containing 5% bovine serum albumin and Tween 20 (PBST) followed by se
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 21-5K 14-3 K - 6-5K - 3-4K Figure 6. Immunoblot of SDS-PAGE of hemolymph from snails (left lane). The hemolymph samples from a mixed population of snails of known growth rates were subjected to SDS-gel electrophoresis and the fractionated proteins were subsequently transferred to nitrocellulose membrane. The membranes were incubated with the primary antiserum (anti-bovine insulin, 1:1000 dilution) for I h, rinsed in phosphate buffered saline, pH , containing 5% bovine serum albumin and Tween 20 (PBST) followed by secondary antiserum conjugated to alkaline phosphatasc (l:IOOO dilution). The membranes were washed and the sites of IgG binding was visualized with nitro blue tetrazolium and 5-bromo-4-chloro-3-indolyl phosphate. Arrows indicate ILPs (16 and 7 kDa) in hemolymph. The molecular weights of the insulin immunoreactive bands were estimated by running the following known molecular weight protein standards (Amersham) on the same gel (right lane): Ovalbumin 46000, carbonic anhydrase 30000, trypsin inhibitor 21500, lysozyme 14300, aprotinin 6500. and insulin (b) chain 3400. incorporation of labeled amino acids into mantle collar and periostrical proteins (P < ; P < , respectively). Furthermore, Figures 10 and 11 demonstrate that addition of the 7-kDa-containing fraction to the culture medium increases incorporation of tritiated amino acids into TCA precipitable proteins of mantle collar and periostracum in a dose-dependent manner up to a dose of 250 ^ILI/ml. Discussion In many mollusks, shell growth is incremental and is influenced by various internal and external factors (Lutz and Rhoads, 1980). The molluscan shell, which includes an organic outer periostracum, is secreted by the mantle collar. Saleuddin and Kunigelis (1984) showed that when the mantle collar was maintained in organ culture, the periostracum formed in vitro was structurally similar to that formed
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
