Practical physiological chemistry : a book designed for use in courses in practical physiological chemistry in schools of medicine and of science . the number of squares, thus obtaining theaverage number of erythrocytes per square. Multiply thisaverage by 4,000 to obtain the number of erythrocytes per i84 PHYSIOLOGICAL CHEMISTRY. cubic millimeter of diluted blood, and multiply this productby ioo or 200, according to the dilution, to obtain the numberof erythrocytes per cubic millimeter of undiluted blood. Thus : Average number of ery-throcytes per square X 4,000 X 200 (or 100) Number of erythr
Practical physiological chemistry : a book designed for use in courses in practical physiological chemistry in schools of medicine and of science . the number of squares, thus obtaining theaverage number of erythrocytes per square. Multiply thisaverage by 4,000 to obtain the number of erythrocytes per i84 PHYSIOLOGICAL CHEMISTRY. cubic millimeter of diluted blood, and multiply this productby ioo or 200, according to the dilution, to obtain the numberof erythrocytes per cubic millimeter of undiluted blood. Thus : Average number of ery-throcytes per square X 4,000 X 200 (or 100) Number of erythrocytesper cubic millimeter. Great care should be taken to see that the capillary pipetteis properly cleaned. After using, it should be immediatelyrinsed out with the diluting fluid, then with water, alcohol andether in the sequence given. Finally dry air should be drawnthrough the capillary and a horse hair inserted to prevent theentrance of dust particles. In counting leucocytes by means of the hsemocytometer pro-ceed as follows: As mentioned above, if the diluting fluid iseither Toisons or Sherringtons solution the leucocytes may Fig. Zapperts Modified Ruling of Thoma-Zeiss Counting Chamber. Costa.) (Da be counted in the same specimen of blood in which the ery-throcytes are counted. When this is done it is customary touse a slide provided with Zapperts modified ruling (Fig. 73,above). This method is rather more accurate than the older BLOOD. is5 one of counting the leucocytes in a separate specimen ofblood. Furthermore it is obviously preferable to count boththe erythrocytes and the leucocytes from the same bl sample. To insure accuracy the number of leucocytes withinthe whole ruled region should be determined in duplicate bloodsamples. This includes the examination of an area eighteen times as great as the old style Thoma-Zeiss central region then would correspond to 3,600 of the smallsquares and if duplicate examinations were made the totalnumber of small s
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