. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. CURRENT AROUND MIGRATING FIBROBLAST 125 ' Figure 4. Clq-induced changes in the extracellular fields derived from L cells migrating into the wound (a-c). Addition of the saline (the Clq solvent) alone induced no response (d). Clq (100 Mg/ml) or the solvent saline was added at the front of centrifugally migrating L cells (at asterisk) at the time indicated by arrows, vp and ret are the same as those in Figures 2 and 3. Note that the extracellular current flowing to the leading front end of migrating fibroblasts increased (t
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. CURRENT AROUND MIGRATING FIBROBLAST 125 ' Figure 4. Clq-induced changes in the extracellular fields derived from L cells migrating into the wound (a-c). Addition of the saline (the Clq solvent) alone induced no response (d). Clq (100 Mg/ml) or the solvent saline was added at the front of centrifugally migrating L cells (at asterisk) at the time indicated by arrows, vp and ret are the same as those in Figures 2 and 3. Note that the extracellular current flowing to the leading front end of migrating fibroblasts increased (the peak amplitude of /lA/crrr) for about 20 s after the brief decrease upon the Clq application. was wounded and thus the cells were found to centripe- tally migrate, the aperture opening above the wound area was always the sink for current (Fig. 3a). In contrast, the opening above the intact part of monolayers was the source, when the circumferential area was scraped away and the cells were found to move centrifugally (Fig. 3b, Fig. 4a). When a complement subcomponent Clq, which is a chemoattractant for L cells and evokes hyperpolarizing responses in the cells (Oiki and Okada, 1988), was ad- ministered in front (but not at the rear: Fig. 4a, bottom trace) of the migrating L cells, biphasic changes in the profile of extracellular fields were observed in all three experiments (Fig. 4). In the saline without cells or on the unwounded monolayer culture, the Clq responses were not observed. Discussion The present data obtained with vibrating probes clearly indicate that extracellular electric currents are en- dogenously associated with fibroblasts migrating into a wound and that the sink for current was concentrated at the leading front end of L cells in motion. Similarly, steady inward currents were observed at the tips of growth cones in retinal ganglion cells (Freeman et al, 1985). Steady currents were also observed around mov- ing amoebas (Nuccitelli et 1977), th
Size: 1803px × 1385px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
