. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. FMRFAMIDE RECEPTORS IN SQUID 189 Table I Inhibition of['25I]-daYFnLRFa binding to SQM by FMRFamide analogs Phe' % INH Mel2 i INH Arg3 % INH Phe4 INH YMRFa FIRFa FMHFa FMRMa HMRFa FFRFa FMSFa FMRIa MMRFa FLRFa FMNFa FMRVa CMRFa FVRFa FMWFa FMRHa AMRFa FYRFa FMAFa FMRAa PMRFa FWRFa FMEFa FMRTa KMRFa FCRFa FMFFa FMRDa LMRFa FTRFa FMKFa FMRLa VMRF
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. FMRFAMIDE RECEPTORS IN SQUID 189 Table I Inhibition of['25I]-daYFnLRFa binding to SQM by FMRFamide analogs Phe' % INH Mel2 i INH Arg3 % INH Phe4 INH YMRFa FIRFa FMHFa FMRMa HMRFa FFRFa FMSFa FMRIa MMRFa FLRFa FMNFa FMRVa CMRFa FVRFa FMWFa FMRHa AMRFa FYRFa FMAFa FMRAa PMRFa FWRFa FMEFa FMRTa KMRFa FCRFa FMFFa FMRDa LMRFa FTRFa FMKFa FMRLa VMRFa FHRFa FMYFa FMRPa NMRFa FQRFa FMPFa FMREa EMRFa FERFa FMTFa FMRKa SMRFa FPRFa FMIFa FMRYa DMRFa FDRFa FMLFa FMRRa QMRFa FRRFa FMGFa FMRNa TMRFa FSRFa FMDFa FMRQa RMRFa FGRFa FMMFa FMRGa FNRFa FKRFa The specific binding of [125I]-daYFnLRFa ( art/) to SQM (15-50 Mg protein) was determined in the presence of a high concentration ( nM, 500 times the IC50 of FMRFamide) of test peptide after incubation for h at 0°C. The percent inhibition of control specific binding (mean, n = 2 or 3) has been displayed for each FMRFamide analog. Values below 50% indicate that the test peptide is more than 500-fold less potent than FMRFamide. trifuged for 25 min at 4,800 X g. The supernatants were decanted and the radioligand binding to antiserum pre- cipitated in the pellets was determined. The nonspecific binding was determined in the presence of 60 pmole FMRFamide; control specific binding (4980 dpm) ac- counted for 91% of the total binding. The inhibition of -o c CQ CJ ^ 'G a 100- 90' 80" 70- 60' 50 40 30 20 10. FMRFa FFRFa FYRFa YMRFa FVRFa FWRFa FHRFa FMHFa FCRFa HMRFa PMRFa FMKFa 10 10' 10' 10 10' 10 10- [Peptide], M Figure 5. Structure-activity relations of peptide binding to FMRFamide recept
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
