. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 128 KIYOZO KAWAI 60 - 50 - 40 • X CD. 10 20 30 40 50 TIME (MINUTES) 60 70 80 FIGURE 2. Effect of carbon monoxide on cytochrome oxidase from pearl oyster gill; ml. extract in each Warburg flask. Final concentration: phosphate buffer at pH , M; ascorbate, M; cytochrome c, X lO"5 M. Final volume ml. I, in 90% N2+ 10% O»; II, in 90% CO + 10% Ck Temperature 25° C. The black and white blocks under the base line show the periods of dark and light. 3. Effect of carbon monoxide on the respiration of lame
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 128 KIYOZO KAWAI 60 - 50 - 40 • X CD. 10 20 30 40 50 TIME (MINUTES) 60 70 80 FIGURE 2. Effect of carbon monoxide on cytochrome oxidase from pearl oyster gill; ml. extract in each Warburg flask. Final concentration: phosphate buffer at pH , M; ascorbate, M; cytochrome c, X lO"5 M. Final volume ml. I, in 90% N2+ 10% O»; II, in 90% CO + 10% Ck Temperature 25° C. The black and white blocks under the base line show the periods of dark and light. 3. Effect of carbon monoxide on the respiration of lamellibranch tissues Inhibition experiments were performed using the same gas mixture (CO/O2 ratio of 9/1) as in the case of cytochrome oxidase. Controls with a gas mixture TABLE II Respiration of lamellibranch tissues in a gas mixture of 90% CO and 10% Oz Animal, tissue No. of deter- minations 02 uptake in control ( 100 mg. fresh wt.) CO inhibition in darkness* (per cent) Relative affinity constant** Crassostrea gigas Heart 2 39 50 Gill 3 53 51 Mantle 2 29 53 Pinctada martensii Gill 4 48 47 Mantle 2 15 54 Digestive diverticula 1 27 46 Mytihis crassitesta Gill 2 26 52 * The complete elimination of CO inhibition by the illumination was observed in all tissues examined. * Relative affinity constant of the tissue respiration for CO and O2 was calculated by the Warburg equation. See Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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