. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 175 185 195 Fraction Number Figure 3. Elution profile of the vanadium-containing substance (vanadob'm)ofAscidiagemmata on SE-cellulose column chromatogra- phy. Vanadium-containing fractions (45 ml) obtained after passing through Sephadex G-15 were loaded onto a column ( cm $ •' 25 cm). After non-absorbed substances were washed off the column by elutmg with 50 mA/ HC1 solution at pH in 5 ml fractions, the vana- dobin was obtained by elution with a linear gradient of KC1 at a concen- tration of A/ and vanadium(IV)
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 175 185 195 Fraction Number Figure 3. Elution profile of the vanadium-containing substance (vanadob'm)ofAscidiagemmata on SE-cellulose column chromatogra- phy. Vanadium-containing fractions (45 ml) obtained after passing through Sephadex G-15 were loaded onto a column ( cm $ •' 25 cm). After non-absorbed substances were washed off the column by elutmg with 50 mA/ HC1 solution at pH in 5 ml fractions, the vana- dobin was obtained by elution with a linear gradient of KC1 at a concen- tration of A/ and vanadium(IV) oxide sulfate (VOSO4) were mixed together at concentrations of mM and mAl, re- spectively, an absorption spectrum closely resembling that of vanadobin after elution through Sephadex G-15 (Fig. 2) was obtained, as shown in Figure 5c. This simula- tion clearly suggested that the vanadobin of.^. gemntata, which waseluted from Sephadex G-15, contained vana- dium in the +3 and +4 oxidation states in the ratio of 45:55, whereas for the purer vanadobin eluting from SE- cellulose, the absorption peak at 410 nm disappeared, and a peak at 756 nm with a shoulder at 620 nm was seen (Fig. 4). These findings indicate that, although vana- dobin originally contains both vanadium forms in the + 3 and +4 oxidation states, vanadium in the +3 oxida- 40 30. 200 250 300 500 600 Wavelength (nm) 700 800 Figure 4. Absorption spectrum of vanadobin eluted from SE-cellu- lose column chromatography. The spectrum was recorded for the peak fraction 205 shown in Figure 3. Absorbance at 238 nm and 756 nm was observed. tion state becomes oxidized to the +4 oxidation state during the purification. Ascidia ahodori When a homogenate containing mg wet weight of the cell pellet was loaded on the Sephadex G-15 col- ( 6. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may no
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