. Culture methods for invertebrate animals;. Invertebrates -- Cultures and culture media; Invertebrates -- Collection and preservation. Calliphoridae 415 experience as may be helpful to other workers. Some of this technique has already been described by us (Minnich, 1929). The procedure to be described has been successful in the culture of the following species: Calliphora erythrocephala, Cynomyia cadaverina, Lucilia sericata, and Phormia regina. The above species will mate and oviposit, and their larvae will mature successfully, at temperatures between 21° and 270 C. At temperatures much belo
. Culture methods for invertebrate animals;. Invertebrates -- Cultures and culture media; Invertebrates -- Collection and preservation. Calliphoridae 415 experience as may be helpful to other workers. Some of this technique has already been described by us (Minnich, 1929). The procedure to be described has been successful in the culture of the following species: Calliphora erythrocephala, Cynomyia cadaverina, Lucilia sericata, and Phormia regina. The above species will mate and oviposit, and their larvae will mature successfully, at temperatures between 21° and 270 C. At temperatures much below or above these limits oviposition is greatly reduced or com- pletely inhibited. A suitable breeding cage housing 50-100 adult flies is a cubicle measuring approximately 25 cm. on each side and consisting of a readily constructed light wooden frame covered with mosquito netting except on the bottom. Cotton tape is used where the gauze is tacked to the frame to insure firmer construc- tion. The wood stock is about 2 x cm. and is smooth. When the mosquito netting has become soiled with the feces and regurgitation of the flies, it may readily be removed and the frame scraped and recov- ered. Since the flies are more or less strongly positive to light the door of the cage should be placed away from the windows of the cul- ture room. To provide additional light and heat we have kept an electric lamp provided with an ordi- nary desk lamp reflector burning close to the lighted side of the cage. A 40-watt frosted bulb is entirely adequate for the purpose. A further device permitting access to the cage and at the same time preventing escape of the flies is provided by a loose curtain of mosquito netting immediately within the door. Paper toweling is a convenient cover for the table on which the cage is placed. Water may be supplied in a funnel (f) from which the delivery tube has been cut and which is inserted into a vial or beaker (v) of the ap- propriate size (Fig. 72). If a bea
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