The Journal of experimental zoology . ichare used as landmarks in experimentation. Consequently, it isimpossible to make exact determinations of the area of limb-forming cells. Location of the limb mesoderm in the absence of surface mark-ings was determined by a fairly accurate method, already de-scribed by the author (Detwiler, 17), a brief description ofwhich may be repeated here. After removing the embryos from the capsule a circular cut SHOULDER GIRDLE AND ANTERIOR LIMB 515 was made just posterior to an imaginary line passing dorso-ventrally midway through the embryo. The ectoderm and meso
The Journal of experimental zoology . ichare used as landmarks in experimentation. Consequently, it isimpossible to make exact determinations of the area of limb-forming cells. Location of the limb mesoderm in the absence of surface mark-ings was determined by a fairly accurate method, already de-scribed by the author (Detwiler, 17), a brief description ofwhich may be repeated here. After removing the embryos from the capsule a circular cut SHOULDER GIRDLE AND ANTERIOR LIMB 515 was made just posterior to an imaginary line passing dorso-ventrally midway through the embryo. The ectoderm and meso-derm were removed. The wound, after being cleaned from allfree mesoderm cells, was then covered over by a circular pieceof ectoderm, cut to fit the excision, taken from an embryo whichhad been properly stained in an aqueous solution of Nile bluesulphate. In about one-half hour this stained disc of ectodermhad healed in and its size and position was then indicated by acamera drawing (text fig. 2). The heavily stippled area represents.
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Keywords: ., bookauthorwi, bookcentury1900, bookdecade1910, booksubjectzoology
