. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 112 SHIGEMI ABE 10-' M PCMB, 5 >: 10-'1 M cysteine and 10~3 M sodium. Under these conditions, the organisms behaved quite normally, and there was no observable effect. In support of this statement it is to be added that the amoebae often phagocystosed ciliated cells in this same medium. For the sake of contrast, a solution containing 5 X 10~5 M PCMB and 10 3 M sodium was used. About two minutes after application, movement of the organisms almost stopped except for the Brownian motion of the granules inside. Three minutes
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 112 SHIGEMI ABE 10-' M PCMB, 5 >: 10-'1 M cysteine and 10~3 M sodium. Under these conditions, the organisms behaved quite normally, and there was no observable effect. In support of this statement it is to be added that the amoebae often phagocystosed ciliated cells in this same medium. For the sake of contrast, a solution containing 5 X 10~5 M PCMB and 10 3 M sodium was used. About two minutes after application, movement of the organisms almost stopped except for the Brownian motion of the granules inside. Three minutes later, Brownian motion stopped; six minutes later, the organism became spherical and giant vacuoles appeared; 25 minutes later, the organism began to cytolyze. Pandorina (1) Immediately after the complete cessation of the flagellar beating with 10^3 M PCMB, the inhibitor solution was replaced with 10~2 M cysteine containing 10~2 M sodium. Then, recovery of the flagellar beating was observed within several min- utes. No abnormal behavior was to be seen later in the organisms which had recovered. Even in the coenobium from which flagella had been partially lost by PCMB, flagella left attached recovered their normal beating. (2) No recovery of flagellar beating took place when 10~3 M PCMB was re- placed with 10 - M NaCl solution. (3) Materials were treated with a mixed solution of 5 X 10'4 M PCMB, 5 X 10~3 M cysteine and 10~2 M sodium. With this solution, there was no visible effect on flagellar beating. On the other hand, when treated with a solution containing 5 X 10~4 M PCMB and 10~- M sodium, beating stopped within a period of 20^1-0 seconds and, some- times, the flagella separated from cell bodies. (4) Simple application of 10~2 M cysteine solution with 10"2 M sodium normal coenobia exerted no TIME IN MINUTES FIGURE 3. Inhibition of the gliding movement of Oscillatoria with PCMB and restoration with cysteine. From 0 to 12 minutes, materials were immersed in 10~3
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
