. Pathological technique; a practical manual for workers in pathological histology and bacteriology. lcium carbonate. With the bouillon cultures so obtainedagglutination tests with immune serum should be carriedout in dilutions of 1 : 100, 1 : 200, 1 : 400. Mixtures ofserum dilutions and bouillon culture of each areplaced in small tubes and incubated at 550 C. for twenty-four hours. Bacillus of Influenza.—Morphology.—Very small ba-cilli, with rounded ends and of variable length, sometimesgrowing into long forms, more or less bent or curved (see Fig- 55). Stains more deeply at the ends
. Pathological technique; a practical manual for workers in pathological histology and bacteriology. lcium carbonate. With the bouillon cultures so obtainedagglutination tests with immune serum should be carriedout in dilutions of 1 : 100, 1 : 200, 1 : 400. Mixtures ofserum dilutions and bouillon culture of each areplaced in small tubes and incubated at 550 C. for twenty-four hours. Bacillus of Influenza.—Morphology.—Very small ba-cilli, with rounded ends and of variable length, sometimesgrowing into long forms, more or less bent or curved (see Fig- 55). Stains more deeply at the ends than in the middle, and inthe long forms shows irregularity of staining. The faintly PATHOGENIC BACTERIA AND FUNGI. 283 stained areas are very sharply defined, as in the case ofthe typhoid bacillus. £ m * « *« » * • , * ♦ :•• •. * *-+ y *« !• • • O ■*** > m m * « • An . ,• is c- **i;. • ..A. 5 •»» • # t* ,>/ y *8 1 •** 2 *• 4 * ; ;* ? • 4M Fig. 56.—Influenza bacilli from a culture on blood-agar ; X 2000 (Wright andBrown).. Fig. 57.—Bacilli of influenza in a leucocyte in a cover-glass preparation fromsputum. A pneumococcus also in the same leucocyte and other pneumococcifree. The small size of the bacillus of influenza will be apparent by comparisonwith the pneumococci; x 2000 (Wright and Brown). In cover-glass preparations from bronchial secretions (seeFig. 56) the bacillus appears smaller and less plump than 284 PATHOLOGICAL TECHNIQUE. it does in preparations from cultures. It also does not showirregularities in staining. Cultivation.—Does not grow in the ordinary culture-media,but maybe cultivated on agar-agar slants, the surfacesof which have been smeared with a few drops of sterileblood. The blood of man, rabbits, guinea-pigs, pigeons, orfrogs will serve for this purpose, the best growth beingobtained with pigeons blood. The blood may be ob-tained from a needle-prick, and spread over the surface
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