. Biological stains; a handbook on the nature and uses of the dyes employed in the biological laboratory. Stains and staining (Microscopy); Stains and Staining; Dyes. Quinone-imine Dyes 129 Moore (1933) has called for phenosafranin in staining colonics of bacteria and fungi. The commercial safranins are ordinarily methyl or ethyl substitution products of this; or occasionally phenyl substitution products. The one of greatest value to the biologist is generally called safranin O. L20 SAFRANIN O C. I. NO. 841 Synonyms: Safranin Y or A. Gossypimine. Cotton red. SHghtly different shades: Safranin
. Biological stains; a handbook on the nature and uses of the dyes employed in the biological laboratory. Stains and staining (Microscopy); Stains and Staining; Dyes. Quinone-imine Dyes 129 Moore (1933) has called for phenosafranin in staining colonics of bacteria and fungi. The commercial safranins are ordinarily methyl or ethyl substitution products of this; or occasionally phenyl substitution products. The one of greatest value to the biologist is generally called safranin O. L20 SAFRANIN O C. I. NO. 841 Synonyms: Safranin Y or A. Gossypimine. Cotton red. SHghtly different shades: Safranin AG, T, MP, and G. (Although all included in C. I. No. 841 they are different from the grade here described.) {A basic dye; absorption maximum about 530) Solubility at 26°C: in water ; in alcohol The common safranins of commerce, under various shade designations, are mixtures of di-methyl and tri-methyl pheno-. C2oHi«N4Cl; Mol. Wt. C21H21N4CI; Mol. Wt. The shade differs according to the proportion of these compounds present, the red being deeper according to the proportion of the tri-methyl compound in the mixture. The type safranin O, which proves best for ordinary biological purposes, can be defined as having its absorption maximum at 530m/x. A spectral curve of safranin O is given in Fig. 16. Safranin has always been a problem, because of its variability, especially in cytological work where it is often employed together with another basic dye, and a delicate balance between the two stains is necessary. The reason for this variation has been a puzzle ever since the Commission has begun its investigations of this dye. The question is not yet answered. It is realized at present, however, that there are samples that stain too blue, and others that stain too yellow, although this difference does not cor- relate with dye content nor with any chemical properties of the dye yet investigated. Strangest to say, there is no difference be- tween any of thes
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