. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. CARBON TRANSLOCATION IN ANEMONES 75 100 - c 80 - S | f 60 - ro O ! "0 -I c °- 20 -. —I— 10 15 —1— 20 I 25 Time (h) Figure 1. The effects of symbiont type and dark chase period on the percent of carbon translocated to the host anemone, n = 2 for each group; ± 1 SD of the mean. translocation) of the labelled carbon in the sample, as de- termined by dividing DPM in the homogenate (or animal) fraction sample (corrected for DPM in the dark control) by the total activity (DPM) of the I4C added and the hours of incubation with
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. CARBON TRANSLOCATION IN ANEMONES 75 100 - c 80 - S | f 60 - ro O ! "0 -I c °- 20 -. —I— 10 15 —1— 20 I 25 Time (h) Figure 1. The effects of symbiont type and dark chase period on the percent of carbon translocated to the host anemone, n = 2 for each group; ± 1 SD of the mean. translocation) of the labelled carbon in the sample, as de- termined by dividing DPM in the homogenate (or animal) fraction sample (corrected for DPM in the dark control) by the total activity (DPM) of the I4C added and the hours of incubation with I4C. The result is the rate of carbon fixation (or translocation), as amount of C fixed (or translocated) per hour. Carbon fixation and translocation rates can be expressed on the basis of both anemone biomass (protein) and on the basis of an individual algal cell. Comparison of rates nor- malized to these two parameters shows how algal density affects photosynthesis and translocation. The rate of carbon fixed by anemones was calculated by using the homogenate fractions in the above calculation and normalizing to either anemone protein biomass or to number of algae. The rate of carbon translocated to the animal was calculated by using the animal fractions in the above calculation. All analyses of variance and multiple range test statistics were examined with a significance level of 5%. Statistics were calculated using Statistix by Analytical Software. Results Percent C translocation over time A I4C pulse-chase time course experiment was conducted with field anemones to determine if and how the length of the dark chase time affected the percent of carbon translo- cated to the host by the two symbionts. A 2 X 6 factorial analysis of variance showed that symbiont type had a sig- nificant effect on percent translocation (P < ). Over the entire chase time period, the percent of fixed carbon translocated to the host by zooxanthellae is significantly higher than
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
