. Applied bacteriology for nurses. Fig. 8.—Media in tubes: a, Broth; 6, agar slant; c, potato (Hiss and Zinsser). mixed with a lot of other bacteria. In other words, itis necessary to obtain a pure culture. We shall assume 27 28 APPLIED BACTERIOLOGY FOR NURSES that we have such a culture; let us now consider themethods by which we continue to grow the first step is to plant the bacteria either in a fluidculture-medium, such as beef-broth, milk, etc., or on the Fig. 9.—Platinum wires for bacteriologic use. surface of a solid medium, such as gelatin, agar, solidi-fied ])lood-serum, pota
. Applied bacteriology for nurses. Fig. 8.—Media in tubes: a, Broth; 6, agar slant; c, potato (Hiss and Zinsser). mixed with a lot of other bacteria. In other words, itis necessary to obtain a pure culture. We shall assume 27 28 APPLIED BACTERIOLOGY FOR NURSES that we have such a culture; let us now consider themethods by which we continue to grow the first step is to plant the bacteria either in a fluidculture-medium, such as beef-broth, milk, etc., or on the Fig. 9.—Platinum wires for bacteriologic use. surface of a solid medium, such as gelatin, agar, solidi-fied ])lood-serum, potato, etc. It is obvious that theseculture-media must be absolutely free from other germs,i. e., they must be perfectly sterile. In planting the. Fig. 10.—Method of holding tubes during inoculation (McFarland). culture, we have at hand the tube containing our originalpure culture and a tube of the sterile medium, e. g.,sterile broth, closed at the top with a cotton plug. Allwe need to do is to transfer a little loopful of culture by CULTIVATIOxX OF BACTERIA 29 means of the platinum wire loop to the tube of sterilebroth. In order to avoid carrying along other germs,however, we first hold the wire loop in the flame untilit glows, thus destroying any germs that may have
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