. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 474 M. DURLIAT AND R. VRANCKX. FIGURE 7. Ouchterlony studies with as antibodies: antiserum (100 /xl) represented by AS; antiplasma (100 /*!) by AP; and anticlottable protein ( /il) by ACP. For the antigens: serum absorbed with anticlottable protein is represented by SI; precipitate line of clottable factor by cf. Other symbols are as in Figure 1. The clottable factor of serum, plasma and coagulable extract gives( a continuous precipitate line showing a complete identity (<-*). In Figure 7c this precipitate line is not
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 474 M. DURLIAT AND R. VRANCKX. FIGURE 7. Ouchterlony studies with as antibodies: antiserum (100 /xl) represented by AS; antiplasma (100 /*!) by AP; and anticlottable protein ( /il) by ACP. For the antigens: serum absorbed with anticlottable protein is represented by SI; precipitate line of clottable factor by cf. Other symbols are as in Figure 1. The clottable factor of serum, plasma and coagulable extract gives( a continuous precipitate line showing a complete identity (<-*). In Figure 7c this precipitate line is not evidenced against SI because remaining clottable factor was absorbed against anticoagulable protein. On each immunodiffusion, two different amounts of S, P and cp are loaded so that variation in density of precipitate line are recorded. borcalis, Hyas coarctatcus (Stewart and Dingle, 1968), nor Oronectes virilis (Wood and Karpawich, 1972). More exactly, it was not detected by electrophoretic analysis. In the last cases plasma and serum proteins were, according to these authors, almost identical. It is possible that the explanation of this difference lies in the quantities of avail- able plasmatic clottable protein which, when these quantities are too low, cannot be detected by electrophoretic analysis. For instance, in Gecarcinus lateralis, plasma and serum electrophoregrams are similar, but hemolymph microscopic observations show the development of fibrin-like strands (Stutman and Dolliver, 1968). Moreover, other observations show that the level of plasmatic clottable factor depends not only on tin- species of the examined animal, but also on its physiological state (intermolt stage, captivity, pathology). Therefore, it is likely that the con-. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biologic
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
