. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. PRODUCTION OF V+ HORMONE BY FAT BODIES 409 FAT BODIES OF PREPUPAE Preliminary experiments indicated that v+ hormone is present in prepupal fat bodies and can be extracted from them during this stage. Several series of extractions of prepupal fat bodies taken from animals of various ages were therefore made. In each case 20 sets of fat bodies were heated in cc. of Ringer's solution and the solution removed by centrifuging through a microfilter. One series, using prepupae from the Oregon-r wild-type stock gave the results
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. PRODUCTION OF V+ HORMONE BY FAT BODIES 409 FAT BODIES OF PREPUPAE Preliminary experiments indicated that v+ hormone is present in prepupal fat bodies and can be extracted from them during this stage. Several series of extractions of prepupal fat bodies taken from animals of various ages were therefore made. In each case 20 sets of fat bodies were heated in cc. of Ringer's solution and the solution removed by centrifuging through a microfilter. One series, using prepupae from the Oregon-r wild-type stock gave the results shown in Table I. TABLE I Age in Hours Number Eye Color, after Puparium of Test Mean and Formation Animals Range 0-1 11 () 14 6-8 10 () 7-9 15 () 10 () There is no apparent reason why the 3^ hour prepupal fat bodies gave negative results. A number of other tests indicate that the results are generally erratic for young prepupae. Thus a separate set gave a mean color value of for an extract of 0-1 hour prepupal fat bodies. A series of tests of prepupae from the Canton-S wild-type stock gave the results shown in Table II. TABLE II Age in hours Number Eye Color larval 10 0-1 10 () 8 () 8 () An additional experiment using 10-12-hour Oregon-r prepupae gave a test of (6 animals ). It should be pointed out that the tests of older prepupae are unreliable because of the impossibility of being sure of getting all of the fat body tissue. At this time the fat bodies are undergoing the breakdown process characteristic of meta- morphosis. These results suffice to show that the hormone is present in fat body tissue and may be extracted over most of the prepupal period. Because of the several difficulties involved in such tests as these, the results are only roughly indicative of quantitative V ^^. Please note that these images are extracted from scanned
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
