. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ULTRAVIOLET AND ARBACIA FERTILIZATION. 429 of the onset of agglutination to he a function of sperm activity. The time during which the agglutinated masses remain intact also (See Fig. 3.) decreases with increased dosage. FIG. 3. Delay in agglutinating reaction following radiation of sperm. Ord:nates, number of seconds elapsed before the onset of agglutination with normal egg-water. Abscissae, length of period of exposure, in seconds. The same conditions are found when egg-water is radiated for a short interval, and th
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ULTRAVIOLET AND ARBACIA FERTILIZATION. 429 of the onset of agglutination to he a function of sperm activity. The time during which the agglutinated masses remain intact also (See Fig. 3.) decreases with increased dosage. FIG. 3. Delay in agglutinating reaction following radiation of sperm. Ord:nates, number of seconds elapsed before the onset of agglutination with normal egg-water. Abscissae, length of period of exposure, in seconds. The same conditions are found when egg-water is radiated for a short interval, and then used to agglutinate normal sperm. Its agglutinating capacity is reduced. Agglutination is delayed, and the masses persist for a shorter period of time than when normal egg-water is used with normal sperm. Longer exposures of either egg-water or sperm, previous to mixture with normal sperm or egg-water, respectively, produce larger irregular permanent masses which " run into each other " forming a kind of reticular mass throughout the suspension. TABLE X. Loss OF AGGLUTINABILITY OF SPERM SUSPENSIONS FOLLOWING RADIATION. A. B. Time Greatest Cone. Greatest Effective Dilution. % Loss. Exp. in Effective Sperm in Sec. Dilution. Per Cent. Non-rad. Rad. o 4,000-8,000 2-5 8,000-16,000 8,000-16,000 — 15 2,000-4,000 4,000-8,000 2,000-4,000 So 30 200-300 i-5 500-1,000 40-80 92 60 20-40 100-200 o-io*abn. 120 • ' A. Sperm in concentration of 3 drops of i per cent, suspension per 10 cc. sea-water. B. Sperm in various concentrations radiated for 30 sec. each. * abn- aggregates were abnormal in appearance, and were permanent and " ;. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass.
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
