. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 340 STEPHENS, VOLK, WRIGHT, AND BACKLUND w "o 1-2 l-O 0-8 0-6 O4 O-2. 2OO 10 50 2OO FIGURE 3. Removal of glycine from solution by Dendraster as a function of ambient con- centrations. The insert graph presents rates determined at the concentrations indicated; the curve is a hyperbola fitted to the kinetic constants. The larger graph is a Woolf plot of the data. Kt is 74 /*M Fmax is 215 nmoles/(hr• cm2). Figure 3 presents data relating influx (measured by disappearance of 14C) to glycine concentration. The insert is a pl
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 340 STEPHENS, VOLK, WRIGHT, AND BACKLUND w "o 1-2 l-O 0-8 0-6 O4 O-2. 2OO 10 50 2OO FIGURE 3. Removal of glycine from solution by Dendraster as a function of ambient con- centrations. The insert graph presents rates determined at the concentrations indicated; the curve is a hyperbola fitted to the kinetic constants. The larger graph is a Woolf plot of the data. Kt is 74 /*M Fmax is 215 nmoles/(hr• cm2). Figure 3 presents data relating influx (measured by disappearance of 14C) to glycine concentration. The insert is a plot of influx as a function of ambient con- centration. Kinetic constants were evaluated from the Woolf plot presented in Figure 3. The Kt was 74 /AM and the Vma.\ was 215 nmoles/(hr-cm2). Dendraster does not take up glycylglycine from dilute solution. Animals were incubated for 24 hours in 20 /AM glycylglycine with a trace amount of 14C-glycine. Radioactivity in the medium decreased rapidly, as expected. Fluorescamine posi- tive material (expressed as equivalent glycylglycine concentration) increased slightly during early incubation, presumably reflecting efflux of unknown primary amines. After 24 hours, levels had decreased to about 75% of the original con- centration. This may reflect microbial activity or very slow uptake. In any case, uptake of glycylglycine either does not occur at all or is so slow as to be insignificant compared to uptake of neutral amino acids. Naturally occurring primary amines in interstitial water were determined using the fluorescamine technique and, in some cases, OPA. Determinations using the. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie,
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
