. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. SYMBIOSIS-SPECIFIC MORPHOGENESIS 2d 3d 349 4d. Figure 2. Time series of CEA regression in symbiotic (sym) and aposymhiotc (apo) juvenile squid over 4 days (d). Symbiotic animals were inoculated with ~ 104 cells/ml of I '.fischeri ES114 within 6 h of hatching. The sym-4d panel represents a fully regressed CEA. Only the right half of each light organ is shown. Scale bar = 100 fim. Differences in size reflect individual variation. Determination of Ciliated Epithelial Appendage (CEA) regression Regression of the CEA was determin
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. SYMBIOSIS-SPECIFIC MORPHOGENESIS 2d 3d 349 4d. Figure 2. Time series of CEA regression in symbiotic (sym) and aposymhiotc (apo) juvenile squid over 4 days (d). Symbiotic animals were inoculated with ~ 104 cells/ml of I '.fischeri ES114 within 6 h of hatching. The sym-4d panel represents a fully regressed CEA. Only the right half of each light organ is shown. Scale bar = 100 fim. Differences in size reflect individual variation. Determination of Ciliated Epithelial Appendage (CEA) regression Regression of the CEA was determined at the end of 4 days for each experiment. Squid were fixed for 24 h in seawater containing either 5% formalin or 2% parafor- maldehyde/2% glutaraldehyde. Samples were subse- quently rinsed twice for 30 min in 50 mA/ sodium phos- phate buffer with M NaCl (pH ), followed by a dehydration series with 15%-100% ethanol. Samples were critical-point dried with liquid CO2, or desiccated with hexamethyldisilazane (Polysciences, Inc.). Dried squid were mounted onto aluminum stubs and the ventral por- tions of the mantle and siphon were dissected away to reveal the juvenile light organs. These samples were then sputter coated with gold and the light organs were ex- amined with a Cambridge 360 scanning electron micro- scope (SEM). The presence or absence of CEA was scored and recorded, and photomicrographs were taken of rep- resentative samples. CEA regression of symbiotic juveniles was compared to that of aposymbiotic juveniles for 4 days following in- oculation with bacteria (Fig. 2). For experiments involving variable exposure times, CEA regression at day 4 was di- vided into five stages (0, 1, 2, 3, and 4), which correspond to the regression seen at 0 (Fig. 1 A), 1, 2, 3, and 4 days of uninterrupted symbiosis (Fig. 2). CEA of light organs were considered regressed if they were at or beyond stage 3. Due to the high variability between individual squid, even within control gro
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
