. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 50 N. W. Figure I. Background-subtracted images of several treated and control colonies of Podocorynecarnea: (A) treated with 30 nM dinitrophenol: (B) dinitrophenol control; (C) fed 6 times per week; (D) feeding control. The genetically identical colonies encrust 15-mm diameter glass cover slips and were imaged at the time of the initiation of medusa production (data from these images are included in Figs. 3 and 6). In each image, polyps are bright and circular, stolons are darker and web-like, and the substratu
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 50 N. W. Figure I. Background-subtracted images of several treated and control colonies of Podocorynecarnea: (A) treated with 30 nM dinitrophenol: (B) dinitrophenol control; (C) fed 6 times per week; (D) feeding control. The genetically identical colonies encrust 15-mm diameter glass cover slips and were imaged at the time of the initiation of medusa production (data from these images are included in Figs. 3 and 6). In each image, polyps are bright and circular, stolons are darker and web-like, and the substratum appears black. Both increased feeding and uncoupling increase the numbers of polyps and encrusting stolons at this developmental landmark; such colonies can be considered peramorphic; / c , they exhibit "shapes beyond" the normal developmental pathways. Clonal replicates of one P. carnea colony were made by explanting 1 -2 polyps and their connecting stolons onto round glass cover slips. For measures of flow rate and morphology, 15-mm diameter cover slips are ideal, but for measures of oxygen uptake and redox state. 12-mm diameter cover slips are necessary. After the explants at- tached and began to grow, all the original explant tissue was removed from the cover slip. In all experiments ex- cept the ones measuring redox state, colonies were effectively confined to one side of the cover slips by cut- ting back encrusting stolons from the reverse side on a daily basis. Cover slips were suspended in floating racks and grown in 120-liter aquaria containing Reef Crystals artificial seawater (salinity == 35%o) with temperature control to ° ± °C, undergravel filtration, and 50% water changes weekly. Ammonia, nitrites, and nitrates were maintained below detectable levels (Aquarium Sys- tems test kits). With the exception of the feeding manip- ulation experiments, colonies were fed to repletion with brine shrimp nauplii 3 days per week. Analysis has shown that, wi
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
