Archive image from page 569 of Currents in biochemical research, 1956;. Currents in biochemical research, 1956; twenty-seven essays charting the present course of biochemical research and considering the intimate relationship of biochemistry to medicine, physiology, and biology currentsinbioche1956gree Year: 1956 ( G. ROBERT GREENBERG Figure 1. Actually the specific activity (and therefore the C activity) of the IMP passes through a rise and fall curve typical of a precursor-product relationship (32). When higher concen- trations of IMP were added to the system and the phosphatase was saturat
Archive image from page 569 of Currents in biochemical research, 1956;. Currents in biochemical research, 1956; twenty-seven essays charting the present course of biochemical research and considering the intimate relationship of biochemistry to medicine, physiology, and biology currentsinbioche1956gree Year: 1956 ( G. ROBERT GREENBERG Figure 1. Actually the specific activity (and therefore the C activity) of the IMP passes through a rise and fall curve typical of a precursor-product relationship (32). When higher concen- trations of IMP were added to the system and the phosphatase was saturated, the rate of conversion of IMP to hypoxanthine could be increased by about 10 times over that observed dur- ing de novo synthesis. T 20 40 TIME, MINUTES Fig. 1. Constancy of an intermediate compound in a steady-state synthesis. It is apparent from the discussion above that in a sequence of enzyme reactions the step with the lowest velocity at saturation with its substrate may not be the limiting reaction in the steady state. Under such conditions, another enzyme may become limiting. SOME ASPECTS SYSTEMS OF THE PURIFICATION OF MULTI-ENZYME Eventually it becomes necessary to fractionate the crude extract in order to isolate intermediate reactants. To discuss 548
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