. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. CHEMORECEPTION IN NASSARIUS 111 (containing fractions 11-18, stippled peak in Fig. 2) was essentially as effective as the total shrimp extract. This conclusion was supported by a Potency Probit Analysis. Three other fractions (101, 104, and 106) were response-inducing at relatively high concentrations. However, the stimulatory capacities of these latter fractions were very low when compared with the capacity of fraction 102. Table II summarizes the distribution of the extract components identified in fraction 102 and in othe
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. CHEMORECEPTION IN NASSARIUS 111 (containing fractions 11-18, stippled peak in Fig. 2) was essentially as effective as the total shrimp extract. This conclusion was supported by a Potency Probit Analysis. Three other fractions (101, 104, and 106) were response-inducing at relatively high concentrations. However, the stimulatory capacities of these latter fractions were very low when compared with the capacity of fraction 102. Table II summarizes the distribution of the extract components identified in fraction 102 and in other preparations obtained by ion exchange separations which were reported previously (for details of separations of shrimp extracts on columns of Rexyn 102 and Dowex 50W-X8, see Carr, 1967). The water effluent from a ) 10 20 30 40 50 60 70 80 90 lOO HO 120 130 140 150 160 FRACTION NUMBER FIGURE 2. Fractionation of a shrimp extract on a column of Dowex SO-X2. Fractions of 6 ml. were collected and aliquots of each were treated with ninhydrin reagent. The optical density at 570 mjt was determined for each aliquot and is plotted as a function of the fraction number. Fractions contributing to peaks were pooled and tested (see Table I). column of Rexyn 102 contained all of the compounds identified in shrimp extracts except "carnitine," trimethylamine oxide (TMO), and lysine. Bioassays had revealed that this effluent possessed a stimulatory capacity comparable to that of the total extract. These results implied that carnitine, TMO, lysine, or other such basic compounds, which might be present in the extract, did not contribute ap- preciably to the stimulatory capacity. The 2 N-NH3 eluate from a column of Dowex 50W-X8 contained all of the compounds identified in the extracts except lactic acid, taurine, and possibly lysine. Bioassays had revealed that this eluate was somewhat less effective than the total extract. The only compounds identified in the water effluent from t
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
