. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. MUSSEL LIM-I RELATED PROTEINS 37 not contain sperm cells and consist of gonad tubules and mantle connective tissue) a very weak 60-kDa immunore- activity was found. At the histological level, not only sperm but also mature Sertoli cells were undetectable in seminif- erous tubules of the spent gonad (Fig. 5B). The results suggest that in mussel male gonad, the 60-kDa antigen is mainly associated with Sertoli cells. This finding is consis- tent with data on Lim-1 protein cell localization in the fetus testis of the rat (Karava
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. MUSSEL LIM-I RELATED PROTEINS 37 not contain sperm cells and consist of gonad tubules and mantle connective tissue) a very weak 60-kDa immunore- activity was found. At the histological level, not only sperm but also mature Sertoli cells were undetectable in seminif- erous tubules of the spent gonad (Fig. 5B). The results suggest that in mussel male gonad, the 60-kDa antigen is mainly associated with Sertoli cells. This finding is consis- tent with data on Lim-1 protein cell localization in the fetus testis of the rat (Karavanov el ai, 1996). The minor 65-kDa band seems to be also characteristic for gonad somatic tissue, although its precise cell association remains to be elucidated. Next we questioned whether the Lim-1 distribution in the M. galloprovincialis female gonad was similar to that in the male gonad. In the female ripe gonad, antibody staining revealed two Lim-1-like antigens with MWs of approxi- mately 65 and 40 kDa (Fig. 6). In the oocyte-free gonad portion, containing mainly collecting tubules and mantle mesenchyme cells, only the 65-kDa fraction was detected. In mature spawned oocytes, both the 65- and the 40-kDa Lim-1 antigens were found. The 40-kDa band does not 12345. v^nM- ,->;-* ••. F '< F &4 B 65- 60- -65 -60 Figure 5. Analysis of Lim-1 antigen distribution in male gonads of A/vr;7ii\ galloprovincialis. Histological sections of ripe male gonad before (Al and after (B) complete spawning (spent gonad). Note that the gonad samples are characterized by the same patterns of follicle (F) morphogen- esis and gonadosomatic index values (in both samples, about of 90% of gonad volume was occupied by follicles) (scale bar—100 jxm). (C) Ex- tracts prepared from complementary gonad (Lane I and Lane 3; arrows) of the same animals, as well as from somatic tissues (Lane 2) and sperm cells (Lane 4) of the other ripe gonad before spawning, were subjected to SDS-PAGE followed by W
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
