. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 186 H. R. KAYE AND H. M. REISWIG C ^ross-absorption tests These tests were performed on antisera 71 and 12, and the results are represented in Figures 1A and 1B. These tests involved absorbing-out an antiserum by incubating various cell numbers of an antigen with the antiserum. When the antigen cell con- centrations were plotted against the antiserum litres an absorption curve was obtained for the homologous antigen (Fig. 1A, #71; and Fig. IB, #12). These curves were used as controls to compare the efficiency of the other an
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 186 H. R. KAYE AND H. M. REISWIG C ^ross-absorption tests These tests were performed on antisera 71 and 12, and the results are represented in Figures 1A and 1B. These tests involved absorbing-out an antiserum by incubating various cell numbers of an antigen with the antiserum. When the antigen cell con- centrations were plotted against the antiserum litres an absorption curve was obtained for the homologous antigen (Fig. 1A, #71; and Fig. IB, #12). These curves were used as controls to compare the efficiency of the other antigens to absorb the antibodies. The results of the cross-absorption tests for sponge antiserum 71 (Fig. 1A) dem- 64 32 16. I o .^71 \ <f 75 50 100 150 20O 3OO CELL COUNT ( x 10° / ml) 64 32 16 B 50 100 15O 200 250 300 CELL COUNT (» 10b /ml) FIGURE 1. A. Results of agglutination test after absorption of a 71 by various sponge cell suspensions. The reciprocal of the dilution (TITRE axis) and concentration of sponge cell suspension (CELL COUNT axis) causing an agglutination reaction to occur are shown. Specimen TA is Verongia fistularis, 67 nd 77 are V. longissima. and 71 and 75 are the same strain (type 3) of V. longissima. B. Results of agglutination test after absorption of a 12 by various sponge cell suspensions. The reciprocal of the dilution (TITRE axis) and concentration of sponge cell suspension (CELL COUNT axis) causing an agglutination reaction to occur are shown. Specimen 77 is V. longissima, and 2 and 12 are the same strain (type 2) of V. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfie
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