. Pharmaceutical bacteriology, with special reference to disinfection and sterilization. Bacteriology. THE MANUFACTURE AND USE OF SERA AND VACCINES. 4. Concentrated Diphtheric Antitoxin. ^33 While the chemical nature of antitoxin is not known, it has been deter- mined that it is united, in some way, with the globulins of the blood. The attempts to isolate antitoxin have resulted in the manufacture of a refined or concentrated antidiphtheric serum which is used quite extensively though it does not meet with the unqualified favor accorded the antidiphtheric serum. The process of manufacture is a
. Pharmaceutical bacteriology, with special reference to disinfection and sterilization. Bacteriology. THE MANUFACTURE AND USE OF SERA AND VACCINES. 4. Concentrated Diphtheric Antitoxin. ^33 While the chemical nature of antitoxin is not known, it has been deter- mined that it is united, in some way, with the globulins of the blood. The attempts to isolate antitoxin have resulted in the manufacture of a refined or concentrated antidiphtheric serum which is used quite extensively though it does not meet with the unqualified favor accorded the antidiphtheric serum. The process of manufacture is as follows:. Fig. 60.—Container with diphtheria antitoxin, supplied with hypodermic needle, piston, all ready for immediate use by the physidan. The plunger is simply a homeopathic vial with rubber stopper. {CutterLaboratory.) A. The antidiphtheric serum is saturated with ammonium sulphate which precipitates the globulins (containing the antitoxin) in the form of a white mass. It is then filtered and the filtrate rejected. B. The precipitate left on the filter is redissolved in water and this solu- tion is again treated with ammonium sulphate as in (a). The object in redissolving in water is to wash the Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Schneider, Albert, 1863-1928. Philadelphia, Blakiston
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