. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. PROTEINS IN THE MORULA-LIKE CELL 145 1212 kDa. 16kDa B Figure 5. Immunoblot analysis and carbohydrate staining of the protein in the morula-like cell and zooxanthellae. (A) Immuno- logical staining; (B) Detection of glycosylated proteins. Lane I, morula- like cell; Lane 2, zooxanthellae. Each lane contained /vg protein. protein but distinct from that of the 17-kDa pro- tein. This suggests that this protein may be synthesized as 16 kDa. After transport into granules, the C-terminal region of the 16-
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. PROTEINS IN THE MORULA-LIKE CELL 145 1212 kDa. 16kDa B Figure 5. Immunoblot analysis and carbohydrate staining of the protein in the morula-like cell and zooxanthellae. (A) Immuno- logical staining; (B) Detection of glycosylated proteins. Lane I, morula- like cell; Lane 2, zooxanthellae. Each lane contained /vg protein. protein but distinct from that of the 17-kDa pro- tein. This suggests that this protein may be synthesized as 16 kDa. After transport into granules, the C-terminal region of the 16-kDa protein may undergo proteolytic maturation to Detection of sugars by the biotin- hydrazide method indicates that 16-kDa and pro- teins are glycosylated. The morula-like cells have been observed in several species of symbiotic clam in the families Tridacnidae and Cardiidae: Tridacna maxima (Reade and Reade, 1976); T. crocea (Nakayama et at., 1997); T. derasa, Hippopus hippopus, H. porcellunas, Corculum cardissa, and Fra- giimfragum (our unpublished observations). The morula- like cell is always observed in the hemolymph of T. cro- cea and constitutes 6% of all the hemocytes (Nakayama et al, 1997). This observation suggests that the morula- like cell may play a role in the giant clam-zooxanthellae symbiosis. Immunodetection showed that the amount of protein immunoreactive to the antibody and of a slightly lower molecular weight (ca. 5 kDa) was far greater in the surface layer of mantle containing zooxan- thellae than in mantle tissue without zooxanthellae. The immunoreactive proteins were also detected in zooxan- thellae and kidney, although their molecular weights were lower than kDa. Some zooxanthellae grown in the zooxanthellal tube may be digested in the stomach or in the siphonal mantle (Maruyama and Heslinga, 1997). Other studies report finding zooxanthellae in the blood vessels of the kidney (Trench et al., 1981) or in amoebo- cytes wi
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