. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. , AIR PRESSURE RELEASE CERCUS INOCULUM LATERAL VIEW. POSTGENITAL PLATE FIGURE 1. Insertion of mosquito into sleeve portion of restraining device. FIGURE 2. Assembly of restraining device. FIGURE 3. Method of drawing abdomen of mosquito through mesh and extension of post- genital region. FIGURE 4. Method for anal injection. Note that malpighian tubules are visible beneath distended pleural membrane. by a nylon mesh (tulle). The sleeve was placed over a snug-fitting post so that a mosquito confined in the sleeve would be press
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. , AIR PRESSURE RELEASE CERCUS INOCULUM LATERAL VIEW. POSTGENITAL PLATE FIGURE 1. Insertion of mosquito into sleeve portion of restraining device. FIGURE 2. Assembly of restraining device. FIGURE 3. Method of drawing abdomen of mosquito through mesh and extension of post- genital region. FIGURE 4. Method for anal injection. Note that malpighian tubules are visible beneath distended pleural membrane. by a nylon mesh (tulle). The sleeve was placed over a snug-fitting post so that a mosquito confined in the sleeve would be pressed against the mesh (Fig. 2). Suction was then applied to the mosquito in order to draw the abdomen through an opening in the mesh (Fig. 3). This resulted in eversion of the terminal abdominal segments and exposure of the anus. A finely drawn pipette was then inserted superficially into the hind gut (free-hand) and fluid expelled from the pipette by means of compressed air ( Fig. 4 ). Resulting distension of the abdomen made it possible to visualize the malpighian tubules which became pressed against the abdominal wall. Occasionally, the gut ruptured and the hemocoele rather than the midgut, filled with inoculum. When this happened, the malpighian tubules floated freely and such mosquitoes were discarded. Artificial jccdint/ Solutions were placed in 2 ml watch glasses and covered with baudrouche mem- brane. The watch glasses were then warmed to 37° C and mosquitoes, confined. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
