. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. I82 OTTO GLASER. equal volume of normal eggs. Both errors really operate against any a priori idea that the jelly is responsible for the removal of the salts. If then the result is clear cut nevertheless, it would appear that the experiment is decisive. Two such comparisons are given in Table V. TABLE V. CHLORINE PER IN TERMS OF AgNO3 n/zo. Sea-Water Sea-Water Sea-Water with with Control- Normal Eggs. Chorion-free Eggs. eggs .2 eggs .2 Alter 30 minutes - = - - = sea-water sea-water
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. I82 OTTO GLASER. equal volume of normal eggs. Both errors really operate against any a priori idea that the jelly is responsible for the removal of the salts. If then the result is clear cut nevertheless, it would appear that the experiment is decisive. Two such comparisons are given in Table V. TABLE V. CHLORINE PER IN TERMS OF AgNO3 n/zo. Sea-Water Sea-Water Sea-Water with with Control- Normal Eggs. Chorion-free Eggs. eggs .2 eggs .2 Alter 30 minutes - = - - = sea-water sea-water eggs .2 eggs .2 After 2 hours = - = sea-water 10 sea-water 10 A further test seemed desirable. If the jelly takes up the salts, it should be possible to demonstrate their presence in the chorion. The method was that of Macallum7 in which the reagent, n/io AgNO3 is acidulated, per liter, with 25 of 60 per cent. HNO3 in order to avoid any confusion that might result from possible phosphate precipitates or combinations of the silver with proteins or their constituent parts. The eggs after being carefully drained were gradually trans- ferred to absolute alcohol, and after hardening, subsequently treated with Macallum's reagent for half an hour. After this they were placed on a glass slide, cleared in glycerine under a cover slip, and exposed to direct sunlight for 30 minutes. The distribution of the reduced silver is shown in Fig. FIG. 2. Arbacia egg with chlorides indicated in chorion. " Macallum, A. B., Die Methoden der Biologischen Mikrochemie. Abder- halden's Handb. d. Bio-chem. Arbeitsmethoden, 1912, p. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ).
Size: 1587px × 1574px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
