. Calcified tissues; proceedings. Bone; Collagen; Calcification. Changes in the Activities of Plasma Acid and Alkaline Phosphatases 183 pattern of the changes in the activities of these enzymes has already been reported (Taylor et ai, 1965), and these changes are shown in Fig. 1. /^c//c changes in the levels ofplosmo Acid and Alkaline phosphatases in relation to egg shell calcification. 3n pre the S 10 /5 JO 0 Hours after start of shell calcificalion changes in the levels of plasma acid and alkaline phosphatases in ss. The points represent mean corrected values for each particular umber of ind
. Calcified tissues; proceedings. Bone; Collagen; Calcification. Changes in the Activities of Plasma Acid and Alkaline Phosphatases 183 pattern of the changes in the activities of these enzymes has already been reported (Taylor et ai, 1965), and these changes are shown in Fig. 1. /^c//c changes in the levels ofplosmo Acid and Alkaline phosphatases in relation to egg shell calcification. 3n pre the S 10 /5 JO 0 Hours after start of shell calcificalion changes in the levels of plasma acid and alkaline phosphatases in ss. The points represent mean corrected values for each particular umber of individual values from which the means are derived are j elation to the eggshell calci- tage of shell formation and en below each point Experimental Ten laying hens were bled at the following times: Sample A — at the time the egg entered the shell gland (determined by regular palpation). Sample B — 4 hours after A, when maximum alkaline phosphatase activity was expected. Sample C — 18 hours after A, when maximum acid phosphatase activity was expected. Sample D — 2 hours after oviposition. Samples A, B and C were assayed for alkaline phosphatase and samples A, C and D for acid phosphatase. The acid phosphatase was determined by the method of Bell and Siller (1962) using M acetate buffer at pH and the alkaline phosphatase according to Bell (1960). Both methods employ disodium p-nitrophenyl phosphate as substrate and the units of activity were calculated in terms of //M substrate hydrolysed per minute per litre plasma. Results The activities of plasma acid and alkaline phosphatase obtained for the individual birds at the 3 stages of the laying cycle are given in Tables 1 and 2 respectively. The marked increase observed in the acid phosphatase values from the time the egg entered the shell gland (A samples) to the time 18 hours later when shell calcification. Please note that these images are extracted from scanned page images that may have been digitally enhanced for
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