. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. FIGURES 5 AND 6. Development of the isolated ventral marginal zone. Fig. 5. Differen- tiation of blood island (in the center), enclosed in the mesothelium, externally covered by the epidermis. Fig. 6. Blood cells, blood vessels, and mesenchyme besides epidermal covering and undifferentiated endoderm. X ca. 60. ponents gradually covered the surface of the endo-mesodermal hemisphere, and four of them fused with each other without losing their original individuality. Twelve days after the operation (culture temperature 21°-26°
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. FIGURES 5 AND 6. Development of the isolated ventral marginal zone. Fig. 5. Differen- tiation of blood island (in the center), enclosed in the mesothelium, externally covered by the epidermis. Fig. 6. Blood cells, blood vessels, and mesenchyme besides epidermal covering and undifferentiated endoderm. X ca. 60. ponents gradually covered the surface of the endo-mesodermal hemisphere, and four of them fused with each other without losing their original individuality. Twelve days after the operation (culture temperature 21°-26° C.), when the large com- bined explant was fixed, it was found attached to the glass bottom with a cylinder of naked endoderm from which protruded a somewhat irregular, elongated body. It was covered completely with the epidermis, half-transparent and expanded. The size of the explant: mm. X mm. Microscopical examination reveals that the protruded part of the explant is filled with loose mesodermal tissues: the yolk-rich mesenchyme, blood vessels enclosing blood cells and the mesothelium lining a wide space which is filled with blood cells (Fig. 6). Within the meshwork of mesenchyme and blood vessels is differentiated a piece of pronephric tubule, with one end attached to the endodermal cylinder. VRK 5. From two gastrulae of stage II the medio-ventral sectors were cut out, fused together and cultured in Holtfreter solution. In the course of two days the explant formed a smaller compact sphere attached loosely to the bottom of the. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 189
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