. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 180 160 140- 120 E Length Width • F Cell Number Surface Area 43,8 37,61. 160 140 ^12° „ 100 "5 o 80 o E 60 CT VI VII VIII Instar Instar Figure 4. Development of the endites. (A, B) Light and fluorescent (for nuclei) images, respectively, of instar VI endite. The setal cells are to the left and the GECs are to the right in each image. Line in B indicates the edge of the region where setae are forming. The arrow indicates a mitotic figure. (C, D) Same adult endite viewed at different focal planes, showing setal cell nucle


. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 180 160 140- 120 E Length Width • F Cell Number Surface Area 43,8 37,61. 160 140 ^12° „ 100 "5 o 80 o E 60 CT VI VII VIII Instar Instar Figure 4. Development of the endites. (A, B) Light and fluorescent (for nuclei) images, respectively, of instar VI endite. The setal cells are to the left and the GECs are to the right in each image. Line in B indicates the edge of the region where setae are forming. The arrow indicates a mitotic figure. (C, D) Same adult endite viewed at different focal planes, showing setal cell nuclei (setal), accessory cells (ac), and GEC nuclei. Bar in A ( = B-D) = 20 (E) Dimensions of the endite during instars V-VIII. Each bar indicates the mean ± I SD of 6-10 larvae. (F) Cell number and mean apical surface area in endite epidermal cells during instars V-VIII. Numbers above bars indicate number of setae and mean length (/urn) of the setae for that instar. Each bar represents the mean ± 1 SD of 4-6 larvae. in instar V to I3l jam in instar VIII and 542 /urn2 by the adult (Fig. 5B. D). Setae began to form during instar VII and increased from four 24-/u,m-long setae in instar VIII to 22 setae with a mean length of 172 in the adult. Tendinal cells formed at least seven muscle attachments during instar V. Apical surface expansion was the primary means of growth in this region. The exopod was first evident as a small bud of 15 cells per surface in instar V (Figs. 2E, 6A). It continued to grow more in length than in width, becoming a thin blade by instar VIII (Fig. 6B, C). It grew from 20 jum in instar V to 51 /urn at instar VIII and to 300 /am in the adult (Figs. ID; 6E). Cell replication increased the cell number from IS in instar V to 30 by instar VIII (Fig. 6F). Ninety GECs were present in the adult. The apical surface area doubled by instar VIII and reached 212 by (he adult (Fig. 6D, F). The GECs became arranged in proximal-distal rows that alterna


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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology