. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. MUSCLE PROTEINS OF APLYSIA 489 1 23456 200 K 116 93 66 45 31 22 14 «* .43 K .35 FIGURE 4. Analysis of thin filaments and actin from Aplysia body wall muscle. Samples were analyzed by electrophoresis in SDS on gradient slab gels of 4-30% polyacrylamide (lanes I-6) or on a 5% polyacrylamide tube gel (lane 7). The molecular weight markers for lane I are the same as for lane 5 of Figure 1; for lanes 2-7 the markers are actin (43,000) and tropomyosin (35,000) from rabbit skeletal muscle. Lane 1: thin filaments (15 ng protein), la
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. MUSCLE PROTEINS OF APLYSIA 489 1 23456 200 K 116 93 66 45 31 22 14 «* .43 K .35 FIGURE 4. Analysis of thin filaments and actin from Aplysia body wall muscle. Samples were analyzed by electrophoresis in SDS on gradient slab gels of 4-30% polyacrylamide (lanes I-6) or on a 5% polyacrylamide tube gel (lane 7). The molecular weight markers for lane I are the same as for lane 5 of Figure 1; for lanes 2-7 the markers are actin (43,000) and tropomyosin (35,000) from rabbit skeletal muscle. Lane 1: thin filaments (15 ng protein), lanes 2-6: fifty ^1 samples of the fractions eluted during DEAE- cellulose chromatography of thin filaments described in the legend to Figure 5; lane 2, fraction no. 39; lane 3, fraction no. 42; lane 4, fraction no. 46; lane 5, fraction no. 49; lane 6, fraction no. 53; lane 7: actin purified from an acetone powder of thin filaments (20 ^g).. cr> E rr, - o cr CL 0 7; o o^ </l -JO 0 20 40 FRACTION NUMBER FIGURE 5. Chromatography of thin filaments from Aplysia body wall muscle on DEAE-cellulose. The column ( X 30 cm) was equilibrated with M KC1 in Buffer D of Gordon et ai, 1976 (10 mM imidazole-Cl (pH ), mA/CaCU, mM ATP, mM beta-mercaptoethanol). 25 ml of Buffer G (same as Buffer D, but with 3 mM imidazole-Cl) was then applied, followed by 50 mg of thin filaments ( mg/ml), and then 25 ml more of Buffer G. The column was then eluted with 125 ml of M KC1 in Buffer D and then a linear gradient (1 liter) of M to M KC1 in Buffer D. Fractions of 17 ml were collected; - —, protein, , concentration of Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
