. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 148 HARRY F. LOWER red by the routine stain. It is non-argentaffin, non-reducing, non-iodophil, and is unaffected by Danielli's, Gibb's, or Mallory's PTAH techniques. It gives negative responses to Sudan black B and Nile blue sulfate. It is easily soluble in hot solutions of potassium hydroxide but resists for at least twelve hours the action of 10% hydrochloric acid at 60° C. These responses indicate a composition largely proteinaceous. The materials to which the protein is bound are such that the usual range of tests do no


. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 148 HARRY F. LOWER red by the routine stain. It is non-argentaffin, non-reducing, non-iodophil, and is unaffected by Danielli's, Gibb's, or Mallory's PTAH techniques. It gives negative responses to Sudan black B and Nile blue sulfate. It is easily soluble in hot solutions of potassium hydroxide but resists for at least twelve hours the action of 10% hydrochloric acid at 60° C. These responses indicate a composition largely proteinaceous. The materials to which the protein is bound are such that the usual range of tests do not serve for their identification. They also confer on it its resistance to extraction bv hot dilute FIGURE 7. Ej>icoccns sp. Dorsal cuticle 4 /*; stained with Sudan black B, bright-field. Hy, hypodermis; L, lipoid zone; mes, mesocuticle. Other symbols as for Figure 1. The Paraffin Layer: This layer is colorless. Its characteristic component is lipoid, as the strong positive responses to fat stains (Fig. 7) and osmic acid indicate. Schmorl's test shows it to possess strong reducing properties and Mallory's PTAH stains it deep violet. It is intensely iodophil but it is not affected by the other reagents used nor is it soluble in dilute acids or alkalis. The Surface Secretion: The dorsal cuticle of living insects or of those killed with cyanide has a resinous luster (Fig. 4) which is retained when the dead specimens are dehydrated. The. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory


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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology