American journal of physiology . tion of 1/98,304 and on eight days was found active in a dilutionof 1/49,152. Comparing these results with the splenectomized ani-mals, we find that on twelve days only was the serum active in a dilu-tion of 1/6144 or over. From the figure we see that the serum of theasplenic animals possessed only one-fourth the titre of the controlsat the height of immunity (1/24,576 versus 1/6144). 2. The rapidity with which the control animals produced the hemo-lysins is very striking when it is compared with the rate of formationby the splenectomized animals which normally
American journal of physiology . tion of 1/98,304 and on eight days was found active in a dilutionof 1/49,152. Comparing these results with the splenectomized ani-mals, we find that on twelve days only was the serum active in a dilu-tion of 1/6144 or over. From the figure we see that the serum of theasplenic animals possessed only one-fourth the titre of the controlsat the height of immunity (1/24,576 versus 1/6144). 2. The rapidity with which the control animals produced the hemo-lysins is very striking when it is compared with the rate of formationby the splenectomized animals which normally laked in a higher dilu-tion. In one instance the rise is distinctly abrupt; in the case of thesplenectomized dogs the rise to the point of maximum concentration ismore drawn out. Fig. 5 shows the same general results as regards the hemagglu-tinins. The more abrupt ascent of the curve of the control animals Relation of Spleen to the Fixation of Antigens. 271 Diln. Days after 7 S 9 10 11 12 13 14 15IG 171819 20 21. Figure 5. — Composite hemagglutinin curve of 9control and 10 asplenic dogs, showing markeddifference in the rate of formation of antibodiesand in the ultimate concentration reached. = 9 control dogs = 10 asplenic dogs. shows how much more rapidly the dogs possessed of a spleen formed the specific agglutinins. On the fifth day the serum of the control animals caused agglutination in a dilution of 1/96. Four days later the serum of the splenecto- mized dogs was found active in a dilution of 1/48. On that day and on the preced-ing day the serum of the control animals had eight times that value. The composite curves of the hemopsonins show the same relationship between the controls and the sple- nectomized animals. A glance at Fig. 6 shows how slowly the asplenic animals formed opsonins. On the eighth day of immunity the serum of the control dogs was at least four times as active as the serum of the splenectomized animals. In many of the individual exper
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