. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 552 KENNEY, BELAMAKICH AND SHEPRO RESULTS Characteristics of aniclwcvtc aggregation in saline When measured photometrically, amebocyte aggregation exhibits the char- acteristics shown in the typical aggregation graph in Figure 1. The sharp decrease in transmission that results from addition of amebocytes to the cuvette is followed by a short lag period, lasting fewer than 15 seconds, in which only a 0-2% rise in transmission occurs. Transmission rapidly rises following this lag period and 80-100 per cent of the total change
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 552 KENNEY, BELAMAKICH AND SHEPRO RESULTS Characteristics of aniclwcvtc aggregation in saline When measured photometrically, amebocyte aggregation exhibits the char- acteristics shown in the typical aggregation graph in Figure 1. The sharp decrease in transmission that results from addition of amebocytes to the cuvette is followed by a short lag period, lasting fewer than 15 seconds, in which only a 0-2% rise in transmission occurs. Transmission rapidly rises following this lag period and 80-100 per cent of the total change in transmission takes place in the first 30 sec- onds. Over the next 30 sec, increases in transmission level off, and during the last 4 min, a plateau occurs where only slight changes in transmission are observed. Amebocyte aggregation can therefore be considered fairly complete at 1 min after I1 60- i. 105 120 TIM E ( sec) FIGURE 2. Typical inhibition of amebocyte aggregation by EDTA and citrate. Control aggregation in saline (• ) ; amebocytes in: EDTA, 17 ±6 mM (O) ; citrate, 17 ± 6 ITIM (A) ; EDTA, 8 ± 3 mM (A) : citrate, 8 ± 3 ITIM ( ©) ; EDTA, 2 ± 1 mM (D) ; citrate, 2 ± 1 mM (•). Concentrations of EDTA and citrate are approximate, but final concentrations in the Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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