. Pharmaceutical bacteriology. Bacteriology; Disinfection and disinfectants. 66 PHARMACEUTICAL BACTERIOLOGY 3. Filling Test-tubes with Culture Media The rule is to pour the culture media hot, although this is not abso- lutely essential. For example, if the media are Uquid in the cool or cold state, as bouillon, serum, milk, etc., they may be poured cold. A good rule is to pour a desired amount of the media just as soon as they are prepared, whether they are still hot or merely warm or cold. Of course, gelatin and agar media must be poured hot or must be liquefied before they can be poured. Fil
. Pharmaceutical bacteriology. Bacteriology; Disinfection and disinfectants. 66 PHARMACEUTICAL BACTERIOLOGY 3. Filling Test-tubes with Culture Media The rule is to pour the culture media hot, although this is not abso- lutely essential. For example, if the media are Uquid in the cool or cold state, as bouillon, serum, milk, etc., they may be poured cold. A good rule is to pour a desired amount of the media just as soon as they are prepared, whether they are still hot or merely warm or cold. Of course, gelatin and agar media must be poured hot or must be liquefied before they can be poured. Fill a small to medium-sized beaker about two-thirds full of the culture medium. Grasp a plugged tube near the upper end, holding it between thumb and first two fingers of the left hand. Remove the cotton plug by. Fig. 15.' -Diagrammatic sectional view of Arnold steam sterilizer illustrating the prin- ciple of steam formation, circulation and condensation. means of the first and second, second and third, or third and fourt^ fingers of the right hand, grasping the free portion of the plug with the back of the fingers toward the cotton. Holding the tube slightly inclined on a level with the mouth, take beaker with medium in right hand (at the same time holding the cotton plug as described), see that the beak rests lightly upon and projects sUghtly over the edge of the tube, and pour, at the same time shifting the eyes to the lower end of the tube to watch the filling process. Fill tubes one-third full. Set down the beaker and replace the cotton plug. Place the filled tubes in special wicker baskets, with a little cotton at the bottom to prevent breaking. Some practice is necessary in order to pour so that none of the liquid comes in contact with the upper third of the tube. This must be avoided, in order to prevent the cotton plug from sticking. Tubes may also be filled from funnel with rubber hose, stop-. Please note that these images are extracted from scanned page images that may
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