. Bacteria : especially as they are related to the economy of nature, to industrial processes and to the public health. Bacteriology; Bacteriology. IMMUNITY AND ANTITOXINS 257 of weakened virulence he would have at hand the necessary " vaccine " for the treatment of the disease. Subsequent research and skilled technique resulted in a method of securing this standard, which he found to be a spinal cord dried for fourteen days. The exact details are as follows : The spinal cords of two rabbits dead of rabies are removed from the spinal canal in their entirety by means of snipping the t
. Bacteria : especially as they are related to the economy of nature, to industrial processes and to the public health. Bacteriology; Bacteriology. IMMUNITY AND ANTITOXINS 257 of weakened virulence he would have at hand the necessary " vaccine " for the treatment of the disease. Subsequent research and skilled technique resulted in a method of securing this standard, which he found to be a spinal cord dried for fourteen days. The exact details are as follows : The spinal cords of two rabbits dead of rabies are removed from the spinal canal in their entirety by means of snipping the trans- verse processes of the vertebrae. Each cord is divided into three more or less equal pieces, and each piece, being snared by a thread of sterilized silk, is care- fully suspended in a sterilized glass jar. At the bottom of the jar is a layer, about half an inch deep, of sterilized calcium chloride. The jars are then removed to a dark chamber, where they are placed at a temperature of 20-22° C. in wooden cases. Here they are left to dry. Above each case is a tube of broth, to which has been added a small piece of the corresponding cord, in order to test for any organismal element that may by chance be included. In case of the slightest tur- bidity in the broth, the cord is rejected. Fourteen series of cords are thus suspended on fourteen consecutive days. The first, second, and third are found to be of practically equal virulence, but from the third to the fourteenth the virulence proportionately decreases, and on the fifteenth day the cord would be practically innocuous and non- virulent When treatment is to be commenced, obviously s. Suspended Spinal Cord In drying jar containing Calcium Chloride. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Newman, George, Sir, 1870-1948. London : John Murray
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