. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 142 C. D. McCAIG AND P. J. DOVER. Figure 2. (a-c) Rhodamine phalloidin labelling of F-actin. (a) Spherical myoball after 6 h at 105 mV/mm. (b) Perpendicularly ori- ented myoblast after h at 150 mV/mm. (c) Elongating myoblast after 30 min at 200 mV/mm. In all cases, F-actin labelling is predomi- nant on the anodal-facmg side of cells (right), (d-f) Rhodamine 123 labelling of live cells to locate mitochondria, (d) Spherical myoball after 5 h at 110 mV/mm. (e) Perpendicularly oriented myoblast after 5 h at 110 mV/mm. (0 Sph
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 142 C. D. McCAIG AND P. J. DOVER. Figure 2. (a-c) Rhodamine phalloidin labelling of F-actin. (a) Spherical myoball after 6 h at 105 mV/mm. (b) Perpendicularly ori- ented myoblast after h at 150 mV/mm. (c) Elongating myoblast after 30 min at 200 mV/mm. In all cases, F-actin labelling is predomi- nant on the anodal-facmg side of cells (right), (d-f) Rhodamine 123 labelling of live cells to locate mitochondria, (d) Spherical myoball after 5 h at 110 mV/mm. (e) Perpendicularly oriented myoblast after 5 h at 110 mV/mm. (0 Spherical myoball after 50 min at 200 mV/mm. In all cases, fluorescence associated with mitochondria is predominant on the anodal-facing side of cells. Scale bar -25 ^m throughout. roof had been applied. This shows that myoblast orienta- tion could occur in cultures that had only been exposed to an electric field for 5-10 min, fully 1 h before most cells had begun to elongate. The effects of exposure to an electric field during peri- ods of suppressed elongation. Microfilament disruption with cytochalasin D (CD) had variable effects on cell ori- entation in an electric field. At mg/ml perpendic- ular orientation was unaffected; at ng/m\ it was inhib- ited, although interestingly elongation still occurred. One ng/m\ completely suppressed all elongation with cells remaining spherical for at least 24 h. Cells were exposed simultaneously to 1 /ug/ml CD, to prevent elongation and to an applied electric field (~ 100 mV/mm) for 4 h. The field was switched off and CD washed out and replaced by normal culture medium. The first cells began to elongate about an hour later and more did so over the ensuing 24 h or so. Such cultures showed considerable degrees of orientation, not disimi- lar to the value found in an electric field alone (Table I. Fig. la). Perpendicular alignment is a calcium-dependent phenomenon and may involve asymmetric distribution of intracellular organelles Wh
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
