. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. SUGAR TRANSPORT IN TAPEWORM 53 Effects of inhibitors In 2 min incubations, 5 mM 2,4-dinitrophenol had no effect on the rate of glucose transport. However, when the worms were preincubated for 10 min in 5 mJl/ dinitrophenol, glucose absorption was inhibited 30% in a subsequent 2 min incubation without dinitrophenol. Sodium iodoacetate at mM produced a 30% inhibition of glucose transport in 2 min incubations. 80 40 600- 200. 20 40 J_ S FIGURE 6. Lineweaver-Burke plots of I4C-glucose absorption in 2-min incubations in the p
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. SUGAR TRANSPORT IN TAPEWORM 53 Effects of inhibitors In 2 min incubations, 5 mM 2,4-dinitrophenol had no effect on the rate of glucose transport. However, when the worms were preincubated for 10 min in 5 mJl/ dinitrophenol, glucose absorption was inhibited 30% in a subsequent 2 min incubation without dinitrophenol. Sodium iodoacetate at mM produced a 30% inhibition of glucose transport in 2 min incubations. 80 40 600- 200. 20 40 J_ S FIGURE 6. Lineweaver-Burke plots of I4C-glucose absorption in 2-min incubations in the presence of galactose (A), maltose (D), a-methylglucoside (A), ouabain (O), or without inhibitor (•). Inhibitors were present at 2 mM. Points for curves with galactose, maltose, and a-methylglucoside as inhibitors are averages of two determinations while those of the ouabain-inhibited and uninhibited glucose absorption curves are individual determinations. Inset is: Curves for absorption of glucose alone (a), glucose in the presence of 2 mM maltose (b), and glucose in the presence of 2 mM ouabain (c). Data points from the lower curves were used to construct inset curves. Abbreviations are: S = mM glucose; V = ^uncles absorbed per gram dry weight per hour. Twenty-eight additional compounds were examined as inhibitors of glucose transport (Table II). Of those tested, eight produced significant inhibitions. When these same compounds were tested as stimulators of the efflux of pre- viously accumulated glucose, only those which inhibited glucose absorption caused an enhanced leakage of glucose. Further, sodium a-ketoglutarate, /3-glycerophos- phate, or dithiazanine had no effect on efflux of previously accumulated Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole,
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
