. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. NOVEL FMRFamide-LIKE PEPTIDE IN HELIX 263 o> «,, •o -o E E O o U- u_ o: o: a> TJ E o u_ cr o Q. LJ _l O. TIME (min) FIGURE 2. A comparison of purified and synthetic FMRFamide-like peptide from Helix aspersa (pQDPFLRFamide) by HPLC. Two runs were made and superimposed. In the first, the trace of ultraviolet absorbance at 257 nm was obtained for 100 nmol of synthetic peptide and is about .005 AU at the peak. In the second run, the distribution of natural peptide in the fractions was determined by radioimmunoassay (RIA) a
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. NOVEL FMRFamide-LIKE PEPTIDE IN HELIX 263 o> «,, •o -o E E O o U- u_ o: o: a> TJ E o u_ cr o Q. LJ _l O. TIME (min) FIGURE 2. A comparison of purified and synthetic FMRFamide-like peptide from Helix aspersa (pQDPFLRFamide) by HPLC. Two runs were made and superimposed. In the first, the trace of ultraviolet absorbance at 257 nm was obtained for 100 nmol of synthetic peptide and is about .005 AU at the peak. In the second run, the distribution of natural peptide in the fractions was determined by radioimmunoassay (RIA) and is expressed in terms of the equivalent quantity of FMRFamide (pmol/fraction); about 180 pmol (by amino acid analysis) in 5 ^1 was injected onto the column (Waters Radial-Pak Micro-Bondapak C-18) which was eluted (4 ml/min) with an ammonium acetate buffer/n-butanol mixture ( M acetic acid and M ammonium acetate; butanol at 80% of saturation); 2 ml fractions were Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
Size: 1224px × 2041px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
