Diagnostic methods, chemical, bacteriological and microscopical : a text-book for students and practitioners . Fig. 151.—Bacillus typhosus at beginning of Widal test. {Da Costa.) sort of culture he has with which to deal. It seems to be fairly well establishedthat a freshly isolated culture is not suitable for diagnostic use, but must betransplanted many times into artificial media. The stock culture of typhoidbacillus is best kept in sealed tubes of nutrient agar in the ice-box, from whichfresh agar cultures should be made every few weeks. In laboratories in which. Fig. 152.—A Pseudo-Widal re
Diagnostic methods, chemical, bacteriological and microscopical : a text-book for students and practitioners . Fig. 151.—Bacillus typhosus at beginning of Widal test. {Da Costa.) sort of culture he has with which to deal. It seems to be fairly well establishedthat a freshly isolated culture is not suitable for diagnostic use, but must betransplanted many times into artificial media. The stock culture of typhoidbacillus is best kept in sealed tubes of nutrient agar in the ice-box, from whichfresh agar cultures should be made every few weeks. In laboratories in which. Fig. 152.—A Pseudo-Widal reaction. {Da Ccsta.) the Widal test is a matter of daily routine, fresh bouillon cultures should bemade daily from the stock cultures, so that one may be certain of havingactively motile organisms at all times. As the general practitioner does not always have facilities at hand for thepropagation of live cultures of bacteria, advantage has been taken of the fact, THE BLOOD. 563 first shown by Widal, that cultures of the bacillus typhosus which had beenkilled by heating to 60 ° C. for 45 minutes did not lose their capacity to aggluti-nate when brought into contact with suspected serum. For this reason, deadcultures of typhoid bacilli, which have been killed by heat or by various chemicalagents, have been introduced for the use of the general worker. The use ofsuch dead cultures does not seem in any way advisable to the writer, as such amethod entirely eliminates the factor of diminished or lost motility, which issuch an important one in deciding as to the c
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