. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 168 S. D. PAINTER. Tissue Extract Added to Bath Figure 2. Secretory products of both the Aplysia califomica albumen gland and atnal gland may be pheromonal attractants. The number of A. brasiliana attracted to a nonlaying conspecific was increased when an extract of either the albumen gland or atnal gland was placed in the adjacent ASW; only the atnal gland significantly altered the pattern of responses. Extracts of other A. califomica reproductive organs did not affect the attractiveness of a nonlaying animal or significant
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 168 S. D. PAINTER. Tissue Extract Added to Bath Figure 2. Secretory products of both the Aplysia califomica albumen gland and atnal gland may be pheromonal attractants. The number of A. brasiliana attracted to a nonlaying conspecific was increased when an extract of either the albumen gland or atnal gland was placed in the adjacent ASW; only the atnal gland significantly altered the pattern of responses. Extracts of other A. califomica reproductive organs did not affect the attractiveness of a nonlaying animal or significantly affect the pattern of responses. This bar graph is based on 105 single-arm expen- ments, 15 per stimulus; in each experiment, animals chose between a stimulus in one arm and no stimulus in the other. Extracts of other reproductive tract organs did not affect the number of animals attracted to the nonlayer or the number of animals making no choice responses (Fig. 2). In each case, the pattern of responses did not differ sig- nificantly from that obtained with the nonlayer alone [X2(2) = , < P < for the mucous gland; X2(2) = , < P < for the winding gland; X2(2) = ; < P < for the RHD; X2(2) = , < P 10 kDa; II. kDa; III, < kDa); each fraction was then distributed into 16 aliquots and lyophilized. The lyophilized aliquots were resus- pended in distilled water, relyophilized, and frozen at —20°C until use. Each was resuspended in 1 ml of distilled water immediately before addition to the maze. Results. Sephadex G-50 elution profiles of acidic ex- tracts of the atrial gland have been published previously (see, , Nagle et a/.. 1988b) and are not shown here. Each of the molecular weight fractions increased the number of animals attracted to the nonlayer and decreased the number of animals making no choice responses (Fig. 3), but only fraction II significantly altered the pattern of responses fr
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