Medical and surgical report of the Presbyterian Hospital in the City of New York . cro-organism which caused the generalinfection. The cut surface was thoroughly seared with a red-hot iron,and a platinum wire of large size was plunged through this surface intothe cortical portion of the kidney parallel with the outer surface, carebeing taken to avoid approaching either the outer surface or the wire was then rubbed thoroughly over the surface of the culturemedium to separate the material as much as possible. The tubes wereplaced in the incubator. On the third day after inoculation,
Medical and surgical report of the Presbyterian Hospital in the City of New York . cro-organism which caused the generalinfection. The cut surface was thoroughly seared with a red-hot iron,and a platinum wire of large size was plunged through this surface intothe cortical portion of the kidney parallel with the outer surface, carebeing taken to avoid approaching either the outer surface or the wire was then rubbed thoroughly over the surface of the culturemedium to separate the material as much as possible. The tubes wereplaced in the incubator. On the third day after inoculation, minutewhite colonies appeared in some of the tubes, and on the fifth day allthe tubes showed from three to ten or twelve similar colonies in colonies increased in size until some of them reached a diameterof one-eighth of an inch, but most of them were smaller. The color,at first white, changed to yellowish-white and then to a decided paleyellow. Having attained a certain size at the base, the colonies ceasedto extend, but became more and more prominent. The growth was. Fig. 4.—A strcjitolhrix tubcn It- in lun^. ( )ritrinal case. 156 STREPTOTHRIX INFECTION. apparently more rapid at the periphery, and the fully developed colonywas round, with convex sides and with a cup-shaded depression at thetop. The height of the colony was sometimes greater and sometimesless than the diameter of the base. The well-developed colonies clingfirmly to the surface of the medium and are not easily detached orbroken up. The growths in all of the tubes were absolutely pure,and consisted of branching threads like those found in the more minute description of these organisms will b& given below. Transplants on Loffler blood-serum produce a pale sulphur-yellowgrowth, forming a layer with a slightly irregular and wrinkled surfaceand prominent edges where the growth continues longest; The colorremains the same until the medium dries, when it becomes white. Thegrowth clings so tenaci
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